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KMID : 1149220160240020100
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Korean Journal of Oriental Medical Prescription
2016 Volume.24 No. 2 p.100 ~ p.107
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Research on Anti-lipogenic Effect of Sobuncheong-eum on Experimental Cellular Model of Non-alcoholic Fatty Liver Disease
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Lee Hye-In
Kim Ji-Soo
Kim Cheon-Joong
Kim Ha-Na
Yang Tae-Jun
Jeong Sang-Jun
Choi Chang-Won
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Abstract
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Objectives : The purpose of this study is to evaluate the anti-lipogenic effect of Sobuncheong-eum on non-alcoholic
fatty liver disease in free fatty acid induced cellular model.
Methods : HepG2 cells were treated with palmitate for 24h to overload intracellular triglyceride (TG) content in the
presence or absence of Sobuncheong-eum extract. After palmitate treatment, Intracellular TG content was measured with TG assay kit. Several lipogenesis related markers, including AMP-activated protein kinase (AMPK), sterol regulatory element-binding transcription factor-1c (SREBP-1c), acetyl-CoA carboxylase (ACC), and fatty acid synthase (FAS), were assessed using Western-blot analyses and RT-PCR.
Results : Palmitate markedly increased intracellular TG in HepG2 cells, and which were alleviated by coadministered
Sobuncheong-eum extract. Sobuncheong-eum extract activated AMPK, which plays a key role in reducing hepatic lipid accumulation, and reduced lipogenic fators, SREBP-1c, ACC, and FAS.
Conclusions : Taken together, it is conceivable that Sobuncheong-eum has an potential to alleviate steatosis, and which may be mediated by activating AMPK at least in part.
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KEYWORD
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Sobuncheong-eum, non-alcoholic fatty liver disease, AMPK, HepG2 cell
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